1. Sebopeho sa lisebelisoa tse tala ka likarolo ka boima ba 'mele: likarolo tse 100 bakeng sa liphoofolo tse ruuoang le nama ea likhoho, likarolo tse 2 bakeng sa metsi, likarolo tse 12 bakeng sa tsoekere, likarolo tse 8 bakeng sa glycerin, le likarolo tse 0,8 bakeng sa letsoai la tafole.Har'a tsona, nama ea liphoofolo tse ruuoang ke khoho.
2. Mokhoa oa tlhahiso:
(1) Tokisetso: Pele ho phekoloa liphoofolo tse ruiloeng le nama ea likhoho ho fumana likotoana tse ’maloa tsa liphoofolo tse ruiloeng le tsa nama ea likhoho;lokisetsa nama ea liphoofolo tse ruiloeng le ea likhoho, metsi, tsoekere, glycerol le letsoai ho ea ka karolelano ea foromo;
(2) Defrosting: khetha liphoofolo tse ruuoang le nama ea likhoho tse batlang li feletse 'me u li behe sebakeng sa 10 ° C hore se qhibilihe ka tlhaho lihora tse 12;
(3) Khaola likotoana: tlosa lesela, letlalo le mafura ho tloha nama e qhibilihisitsoeng ka ho feletseng le likhoho, 'me u khaole likotoana ho fumana liphoofolo tse ruiloeng le nama ea likhoho;sebopeho sa nama ea liphoofolo tse ruiloeng le ea likhoho ke hlobolisa, sekoere, taemane, khutlotharo kapa libopeho tse ling
(4) Ho Hloekisa: Kenya liphoofolo tse sehiloeng le nama ea likhoho metsing a hloekileng ’me u li hlatsoe hape, u li inele metsing a phallang ka metsotso e 20;
(5) Ho ntša metsi: Beha liphoofolo tse hlatsoitsoeng le nama ea likhoho holim'a terei ea ho ntša metsi, 'me u ntše metsi ka metsotso e 60 ka 5 ℃;
(6) Tumble: Beha foromo ea palo ea liphoofolo tse ruiloeng le nama ea likhoho ka har'a tumbler, ebe u eketsa palo ea metsi, tsoekere, glycerin le letsoai;bulela tumbler hore e theohe ho fumana motsoako oa pele oa liphoofolo tse ruuoang le nama ea likhoho;taolo Mekhahlelo ke e latelang: Ka mor'a hore kneader ea tumble e tlosoe ho -0.06Mpa, ka lebelo la 60r / min, e tla potoloha pele ka metsotso e 10 ebe e khutlela morao ka metsotso e 10;
(7) E eme: Kenya motsoako oa pele oa nama ea liphoofolo tse ruiloeng le ea likhoho ka har'a setshelo 'me u e tlohelle e eme ho -8 ° C bakeng sa lihora tse 4 ho fumana motsoako oa bobeli oa liphoofolo le nama ea likhoho;
(8) Beha sejana le ho se halika: Beha motsoako oa bobeli oa nama ea liphoofolo tse ruiloeng le ea likhoho holim’a terei ea letlooa, ebe u e beha ka phaposing ea ho omisa bakeng sa ho omisoa.Mocheso oa ho omisa ke 45 ° C mme nako ea ho omisa ke lihora tse 6.Motsoako o motle oa boraro oa mehlape le nama ea likhoho;
(9) Cooling: pholiso ea boraro ea liphoofolo tse ruuoang le likhoho nama ea motsoako ka mocheso o tloaelehileng le tikoloho e omileng ho fumana motsoako oa bone oa liphoofolo le nama ea likhoho;mocheso o pholileng ke 30 ° C, mongobo oa moea ke 40%, 'me nako ea ho pholile ke lihora tse 6;
(10) Ho qhoqhoa ka potlako: Kenya motsoako oa bone oa nama ea liphoofolo tse ruiloeng le ea likhoho sebakeng sa polokelo se batang ka potlako bakeng sa ho hatsetsoa ho fumana motsoako oa bohlano oa liphoofolo tse ruuoang le nama ea likhoho;mocheso oa serame -40 ° C, nako ea serame lihora tse 8;
(11) Ho omisa ka leqhoa: Beha motsoako oa bohlano oa liphoofolo tse ruuoang le nama ea likhoho ka moqomong o omisang ka serame bakeng sa ho omisoa ho fumana lijo tsa liphoofolo tse ruuoang ke leqhoa tse omisitsoeng.Nako ea lyophilization ke lihora tse 20, 'me mocheso oa lyophilization ke -50 ° C.
(12) Ho lemoha ka tšepe: Beha lijo tse omisitsoeng tse omisitsoeng ka leqhoa holim'a terei ea marang-rang, 'me u khethe lihlahisoa tse nang le likarolo tsa tšepe ka mochine oa tšepe;lisebelisoa tsa ho lemoha tšepe Fe: 2mm, SuS: 1mm;
(13) Ho paka: Sebelisa mochini oa vacuum bakeng sa sephutheloana sa vacuum, degree ea vacuum -0.04MPa.
(2) Ho qhoqhoa ka potlako.Beha sampole ka sehatsetsing se potlakileng 'me u hatsetse ho -18 ° C.
(3) Ho baka.Tlosa thepa, e behe ka har'a terei ea ho baka, 'me u e romele ka ontong.(Ho ea holimo le tlase mollo, chesa ka 150 ℃ bakeng sa metsotso e 5, ebe u fetola 130 ℃ bakeng sa 10min).Hlatsoa mahe a linotši a lokiselitsoeng ka metsi holim'a nama e bolokiloeng 'me ue romele ka ontong hape (ho ea holimo le ho theosa mollo, 130 ℃, 5min).E ntše, e koahele ka lesela la pampiri e tlotsitsoeng, e fetole holim'a terei ea ho baka, borashe ka metsi a mahe a linotši, 'me qetellong ue romele ka ontong (ho ea holimo le ho theosa le mollo, 130 ℃, 20min e ka tsoa ka ontong).Khaola nama e halikiloeng ka sebopeho sa khutlonnetsepa.
Nako ea poso: Aug-22-2020